Isolation of Intact RNA Following Cryosection of Archived Frozen Tissue

Isolation of intact RNA following cryosection of archived frozen tissue.

Advances in our understanding of the expression of particular genes during the course of various human diseases have led to an increasing need to obtain high-quality and intact RNA from tissue specimens to facilitate functional genomic investigations. The emergence of laser microdissection microscopy technology has allowed researchers to undertake such studies on specific cell populations consi...

Texture Analysis and Tissue Segmentation of Cryosection Images

This paper outlines the exploration of two methods to detect texture in a digital cryosection image from the Visible Human Project. For the purpose of this research, texture is defined as a regular or irregular placement of color in an image. A higher-level decision-making algorithm was employed to extract different body tissues: fat, muscle, and bone. This algorithm was designed on the premise...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

inductive effect of palatal connective tissue on alveolar mocosa after subepithelial connective tissue graft clinical & histologic study

چکیده ندارد.

Optimization of RNA Extraction from Rat Pancreatic Tissue

Background: Optimized RNA extraction from tissues and cell lines consists of four main stages regardless of the method of extraction: 1) homogenizing, 2) effective denaturation of proteins from RNA, 3) inactivation of ribonuclease, and 4) removal of any DNA, protein, and carbohydrate contamination. Isolation of undamaged intact RNA is challenging when the related tissue contains high levels of ...